Current doctoral students

Narendran.Annadurai01@upol.cz's picture

Narendran Annadurai

Supervisor: Das Viswanath M.Sc., Ph.D.
Status: Ongoing
Specialization:

Tau aggregation inhibitors in the treatment of neurodegenerative diseases

Understanding the mechanism of tau aggregation, propagation and cellular machinery involved in aggregated tau degradation would help in identifying therapeutic targets for tauopathies (Alzheimer’s disease, PSP, CTE, FTLD, etc.) and other neurodegenerative diseases. The thesis is focused on studying tau aggregate spreading mechanisms, identifying small molecule inhibitors of tau misfolding and aggregation using in vitro aggregation assays, cell biology, biochemistry and microscopy techniques.
barbora.manisova@upol.cz's picture

Barbora Hošíková

Supervisor: Binder Svatopluk Ph.D.
Status: Ongoing
Specialization:

In vitro study of the effect of the photosensitive substance on the tumor cell lines

This study deals with utilization of newly synthetised photosensitizers chemically clasified as phthalocyanines in photodynamic therapy.The photosensitizers are tested in vitro on tumor cell line – HeLa (cervical cancer cells) and non-tumor cell line NIH3T3 (mouse fibroblasts). A main goal is to find the photosensitizer causing the highest phototoxic effect on the tumor cells with minimal side effects.
alzbeta.chabronova01@upol.cz's picture

Alžběta Chabroňová

Supervisor: Navrátilová Zdenka Ph.D.
Status: Ongoing
Specialization: Physiology and pathophysiology

Exosomal miRNAs in chronic obstructive lung disease

This study will be focused on identifying mechanisms of post-transcriptional regulation in COPD pathogenesis by measuring expression of selected miRNAs and determining differentially expressed miRNAs in patients vs controls.
jaroslava.jacova01@upol.cz's picture

Jaroslava Jáčová

Supervisor: Adam Tomáš Ph.D.
Status: Ongoing
Specialization:

Metabolomic GC×GC-TOF MS analysis of biofluids

The thesis is focused on metabolomic analysis of biofluids, using comprehesnive two-dimensional gas chromatography coupled with time of flight mass spectrometry (GC×GC-TOF MS). The optimal column combination suitable for given biofluid has been evaluated, GC×GC TOF-MS analysis has been optimized, separation efficiency has been determined, data processing method has been developed.
hana.jaworek@upol.cz's picture

Hana Jaworek

Supervisor: Koudeláková Vladimíra PhD.
Status: Ongoing
Specialization:

Human papillomavirus and its significance for diagnostics and therapeutics

The thesis is focused on influence of HPV infection on fertility and cancer development. HPV DNA is detected by PCR based diagnostics and in situ hybridisation. The main goal is to determine an influence of HPV on fertility and cancer development.
ja.horakova@upol.cz's picture

Jana Jiravová

Supervisor: Bartoň Tománková Kateřina Ph.D.
Status: Ongoing
Specialization:

The study of nanomaterials using modern microscopic techniques

The thesis is focused on the characterisation of nanoparticles properties and detection effect of nanoparticles on biological systems included tumor and non-tumor cell lines. For detection of nanoparticles are used modern microscopic techniques (e.g. atomic force microscopy, Raman spectroscopy, confocal and electron microscopy). Nanoparticles are massively used in daily life, so it is important to know their properties.
lenka.kocourkova@upol.cz's picture

Lenka Kocourková

Supervisor: Smešný Trtková Kateřina Ph.D.
Status: Ongoing
Specialization:

Effect of coregulators on the androgen receptor activity in cells derived from prostate cancer after their treatment with histone deacetylase inhibitor

The thesis is focused on the detection of coregulator expressions in cancer and normal prostate cells. Coregulators, coactivators as well as coregulators, play an important role in androgen receptor-mediated progression of the prostate cancer. There is a competition between coactivators and corepressors for binding on the androgen receptor and therefore, the changes and the expression ratio between could be important for prostate cancer growth and progression. We focus on two regulators: SMRT (silencing mediator for retinoid and thyroid hormone receptors) as corepressor, and p300 as coactivator.
jana.kotulova@upol.cz's picture

Jana Kotulová

Supervisor: Džubák Petr M.D., Ph.D.
Status: Ongoing
Specialization:

Adenosine receptor inhibitors, the development and optimization

Adenosine receptors are involved in several cellular processes and can play significant role in development of various diseases, including cancer. The aim of the thesis is to study the role of adenosine receptors in cell physiology as well as in pathological state. This work is focused on screening of potential ligands of adenosine receptors and characterization of their molecular interactions using high-throughput screening approach and further validation by methods of molecular biology. Novel potent inhibitors can be beneficial for cancer therapy in the future.
natalie.kudlova@upol.cz's picture

Natálie Kudlová

Supervisor: Hajdúch Marián M.D., Ph.D.
Status: Ongoing
Specialization:

High throughput sequencing methods in biomarker identification and validation

High throughput sequencing is rapidly improving in its quality, speed and cost. This scientific approach increases our ability to characterise diseases at the genomic, transcriptomic and epigenetic levels. The aim of the thesis is to use this technique for studying cells under ribosomal stress. The work is focused on the identification and validation of biomarkers that are in connection with ribosomal stress caused diseases.
zuzana.maceckova01@upol.cz's picture

Zuzana Macečková

Supervisor: Hajdúch Marián M.D., Ph.D.
Status: Ongoing
Specialization:

Molecular pathology of Diamond-Blackfan anemia

Diamon-Blacfan anemia is a rare congenital red cell aplasia, often associated with congenital malformations, particularly of upper limbs and craniofacial region. As a cause, mutations in genes for ribosomal proteins have been reported in approximately 50% of the cases. Mutations in ribosomal proteins lead to defects during ribosome biogenesis and thus ribosome stress, which in turn leads to cell cycle arrest or apoptosis in erythroid progenitors.In my study, I focus on discovering and validation of new possible molecular causes of Diamond-Blackfan anemia and other ribosomopathies by NGS approach followed by functional studies of chosen genes.
lucie.madrova@upol.cz's picture

Lucie Mádrová

Supervisor: Adam Tomáš Ph.D.
Status: Ongoing
Specialization:

Study of the effect of selected cancerostatics on the cell metabolome

The thesis is focused on the development of both targeted and untargeted metabolomics methods (liquid chromatography coupled with high resolution mass spectrometry) for the study of the effects of various anticancer drugs on model cell lines. The main goal is to quantitatively analyse changes in metabolome’s composition for the comprehensive characterization of metabolic networks.
zuzana.mala@imtm.cz's picture

Zuzana Malá

Supervisor: Kolářová Hana Ph.D.
Status: Ongoing
Specialization:

Nanomaterials and the study of cell damage fluorescence methods

The thesis is focused on the study of effect of various porphyrin and phtalocyanin photosensitizers during different light intensity on cell and bacterial lines using fluorescent, molecular and microscopis methods. Further the thesis is focused on the characterisation of nanoparticles properties combinated with photodynamic therapy.
lukas.malina01@upol.cz's picture

Lukáš Malina

Supervisor: Bartoň Tománková Kateřina Ph.D.
Status: Ongoing
Specialization:

Study of cell destruction using physical and chemical methods

The thesis is focused on the study of effect of various porphyrin and phtalocyanin photosensitizers during different light intensity on G361 (human skin melanoma) and NIH3T3 (mouse fibroblasts) cell lines using fluorescent, molecular and microscopic methods. Main aim is on the dependence of photodynamic therapy on photosensitizer type, its concentration and light doses on tumorous and non-tumorous lines to choose best fitting photosensitizer.
mariia.matveieva@upol.cz's picture

Mariia Matveieva

Supervisor: Polishchuk Pavlo Ph.D., M.Sc.
Status: Ongoing
Specialization: Pediatrics

Automatic mining of relationships between structure and activity in chemical databases

The thesis is focused on the development of methods and tools for mining valuable structural features of compounds in chemical databases. Those methods are being developed with the main goal to explain observed structure-activity relationships and to design new molecules with improved properties.
martina.michalova01@upol.cz's picture

Martina Medvedíková

Supervisor: Mašek Vlastimil Ph.D.
Status: Ongoing
Specialization:

Study of interaction of biologically active small molecules with selected target macromolecules

The thesis is focused on the study of the interaction potential anticancer drugs with target macromolecules (DNA, proteins). These interactions are analyzed using various methods, such as isothermal titration calorimetry, confocal Raman microscopy and molecular biology methods.
e.novosadova@upol.cz's picture

Eva Novosadová

Supervisor: Petřek Martin M.D., CSc.
Status: Ongoing
Specialization:

Role of RBPs (RNA- binding proteins) in regulation of immune reactions

In this thesis will be studied RNA- binding proteins on their expression level. Further will be investigated their possible role of biomarkers in patients with inflammatory lung diseases.
hanus.slavik@upol.cz's picture

Hanuš Slavík

Supervisor: Srovnal Josef M.D., Ph.D.
Status: Ongoing
Specialization:

Minimal residual disease in solid cancers

The main goal of this thesis is to discover new biomarkers on the level of RNA. These biomarkers can be tested for usage in predictive and prognostic medicine and they can help in early diagnostic or in postoperative identification of the risk of recurrence. The methods used for this purpose are RT-qRCR and microarray assays (cytoscan).
jarmila.stankova@upol.cz's picture

Jarmila Stanková

Supervisor: Džubák Petr M.D., Ph.D.
Status: Ongoing
Specialization:

Cytotoxic drugs molecular target identification by methods of cell biology and proteomics

Molecular target identification of biologically active molecules is a key factor facilitating rational design and preclinical development of potential drugs. Methods of cellular biology, chemical proteomics and mass spectrometry are developed and used for its purposes.
zdenek.skrott@upol.cz's picture

Zdeněk Škrott

Supervisor: Mistrík Martin Ph.D.
Status: Ongoing
Specialization:

The role of the ubiquitin-proteasome system in tumorigenesis and cancer treatment

Regulated protein degradation is mediated by ubiquitin-proteasome system. Inhibition of proteasome proteolytic activity is a promising approach in cancer therapy with first-in-class drug bortezomib approved for the treatment of patients with multiple myeloma. Since ubiquitination regulates not only protein degradation but also wide variety of cellular signaling pathways, this new approach might bring new drugs with better efficacy and safety profiles.
zuzana.sporikova@upol.cz's picture

Zuzana Šporiková

Supervisor: Trojanec Radek Ph.D.
Status: Ongoing
Specialization:

Cytogenetics changes in solid tumors

The thesis is focused on the identification of molecular and genetic changes in solid tumors focusing on glial tumors especially on a group of recurrent glioblastoma. The main goal is to identify molecular and genetic aberrations which are responsible for glioblastoma progression and recurrence by comprehensive, complementary molecular genetic methods and high-throughput technologies.
zsofiaturi's picture

Zsofia Turi

Supervisor: Moudrý Pavel Ph.D.
Status: Ongoing
Specialization:

Replication stress induced cellular senescence in primary fibroblast cell lines

Cellular senescence is defined as a permanent cell cycle checkpoint arrest. The two tumor suppressor proteins indicated in cellular senescence, p16INK4a and p14ARF are encoded on the same locus, although activated independently from each other, as a consequence of various stresses. My study focuses on the identification of the kinetics and mode of their activation upon replication stress.
j.vaclavik@upol.cz's picture

Jan Václavík

Supervisor: Adam Tomáš Ph.D.
Status: Ongoing
Specialization:

Metabolomic tools for diagnosis of inherited metabolic disorders

Inherited metabolic disorders of purine and pyrimidine (PP) metabolism exhibit broad neurological, hematological, immunological and renal manifestations. They are characterized by increased concentrations of PP metabolites in body fluids caused by abnormal or missing activity of particular enzyme which is involved in their metabolism. Liquid chromatography coupled to tandem mass spectrometry (LC‐MS/MS) is method of choice in the last 15 years for diagnoses of PP metabolic disorders. Due to instrumental price drop high‐resolution mass spectrometry (HRMS) is starting to be increasingly used in clinical laboratories. The aim of this work is to compare diagnostic efficacy of these two approaches.
jana.vaclavkova02@upol.cz's picture

Jana Václavková

Supervisor: Džubák Petr M.D., Ph.D.
Status: Ongoing
Specialization:

Identification of mechanisms of action of molecules with anticancer effect

The thesis is focused on optimization of the method for identifying cellular targets of anticancer drugs. The aim of the work is to optimize affinity purification using streptavidin coated plates. Next the new drugs will be tested to find their molecular target and the validation studies will be performed.
monika.vidlarova@upol.cz's picture

Monika Vidlařová

Supervisor: Srovnal Josef M.D., Ph.D.
Status: Ongoing
Specialization:

Detection and clinical importance of circulating tumor cells in solid tumours

The thesis is focused on the detection of circulating tumour cells (CTCs) by different approaches and the clinical importance of their presence in solid tumours, especially in lung, pancreatic and colorectal cancer patients. The main goal is to identify patients with high risk of disease recurrence and in need of adjuvant therapy. The second goal is to participate in the research and development of novel methods of CTCs detection and characterization.
ludmila.zarska@imtm.cz's picture

Ludmila Žárská

Supervisor: Kolářová Hana Ph.D.
Status: Ongoing
Specialization:

Photodynamically active substances possibility of their use for photodynamic therapy of tumors

The thesis is focused on the study of effect of various porphyrin and phtalocyanin photosensitizers during different light intensity on cell lines using molecular and microscopic methods.