Light source with luminous field especially for inducing and monitoring photodynamic phenomenon in vitro
Photodynamic therapy (PDT) is besides chemotherapy, radiotherapy and immunotherapy other option for treatment of tumor diseases. It is a kind of photochemotherapy that is characterized by a harmful effect of photodynamically active drug and light in the presence of oxygen molecules. The physical principle of PDT is energy or electron transfer from excited sensitizer onto an oxygen molecule or other substrate. This transfer is accompanied by reactive oxygen species (ROS) or free radicals formation. Due to fast photophysical and chemical reactions related to ROS production, it is necessary to monitor these processes continuously. Our light source was proposed to provide homogenous light sample irradiation with continuous monitoring of products formation during PDT by means of common spectrophotometers or readers.
The invention is in an arrangement of a light source with homogeneity of light field, primarily for induction and monitoring of photodynamic effect in vitro, intended primarily for treatment tumor diseases via photodynamic therapy.
Prototype. Validation protocols.
Light source with homogeneity of light field, primarily for induction and monitoring of photodynamic effect in vitro consists of a body adapted for sample placing and a set of LED diodes. LEDs are mounted inside of the body and arranged in a circle around the sample minimally in one horizontal plane so that their light beams are focused on the sample. LED diodes are exchangeable.
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Krestyn, E., H. Kolarova, R. Bajgar, K. Tomankova. Photodynamic properties of ZnTPPS4, ClAlPcS2 and ALA in human melanoma G361 cells. Toxicology in Vitro. 2010, 24(1), 286-91. ISSN: 1879-3177. IF: 3.105. PMID: 19720133 Kolarova, H., R. Bajgar, K. Tomankova, E. Krestyn, L. Dolezal, J. Halek. In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells. Physiological Research. 2007, 56, S27-32. ISSN: 0862-8408. IF: 1.324. PMID: 17552898
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc
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