Koberna Karel CSc.

EDUCATION

1989 – 1994     Title CSc. (equivalent of PhD), Institute of Experimental Medicine AS CR

1983 – 1988     Title MSc. and RNDr. (Rerum naturalium doctor), Faculty of Science, Charles University in Prague; Specialization – Molecular Biology

Area of interest, the basic used technologies and special knowledge

- The effect of the precursors of nucleic acids on their metabolism and cellular metabolism, the development of the new methods for the detection of proliferation activity of mammalian cells and systems for the fast detection of proteins based on the magnetic separations, organisation of chromatin, DNA replication and repair, the development of the new methods for measurement of enzyme activity, study of base excision repair and its use in cancer treatment.

- Immunocytochemical detections, confocal and light microscopy, molecular biology methods, cell cultivation, electron microscopy including electron tomography, high pressure freezing and freeze substitution.

- Other knowledge: work with Microsoft Office, Adobe Photoshop, Corel Draw, ImageJ, Cell Profiler, ilastik, 3D model programs: Rhinoceros, programming in Python.

PUBLICATIONS

1. Ligasová A., et al. (2023) Int J Mol Sci 24(4): 3674
2. Hodoň J., et al. (2022) Eur J Med Chem 243: 114777
3. Ligasová A., et al. (2021) Open Biol 11(10): 210136
4. Ligasová A., Koberna K. (2021) Int J Mol Sci 22(19): 10759
5. Ligasová A., Koberna K. (2021) Molecules 26(18): 5515
6. Ligasová A., Koberna K. (2021) Int J Mol Sci 22(13): 7098
7. Ligasová A., Koberna K. (2021) Chapter 17, pp 111-120, In Laboratory Techniques in Cellular and Molecular Medicine, Agrawal, K., Bouchal, J., Das, V., Drábek, J., Džubák, P., Hajdúch, M., Koberna, K., Ligasová, A., Mistrík, M., de Sanctis, J.B., et al., Eds. Palacký University Olomouc: Olomouc.
8. Ligasová A., Koberna K. (2021) Chapter 18, pp 121-132, In Laboratory Techniques in Cellular and Molecular Medicine, Agrawal, K., Bouchal, J., Das, V., Drábek, J., Džubák, P., Hajdúch, M., Koberna, K., Ligasová, A., Mistrík, M., de Sanctis, J.B., et al., Eds. Palacký University Olomouc: Olomouc.
9. Ligasová A., et al. (2019) Cells, 8(12): E1510
10. Ligasová A., Koberna K. (2019) Sci. Rep., 9(1): 8701
11. Ligasová A., Koberna K. (2018) Molecules, 23(11): 3007
12. Ligasová, A., et al. (2017) PLoS One 12(4): e0175880
13. Ligasová, A., et al. (2017) PLoS One 12(3): e0174893
14. Ligasová, A., et al. (2016) Open Biol, 6:150172
15. Ligasová, A., Koberna, K. (2015) Methods Mol. Biol., 1351:81-92, M. McKenzie ed.
16. Ligasová, A., et al. (2015) PLoS One 10(7): e0132393
17. Ligasová, A., et al. (2015) PLoS One, 10(2): e0117459
18. Cmarko, D., et al. (2014) Methods Mol. Biol., 1117:343-66, John Kuo ed.
19. Ligasová, A., et al. (2013) PLoS One, 8(6):e66864
20. Ligasová, A., et al. (2012) PLoS One, 7(12):e52584
21. Liboska, R., et al. (2012) PLoS One, 7(12):e51679
22. Ligasová, A., et al. (2011) Parasit. Vectors, 4:162
23. Ligasová, A., Koberna, K. (2011) DNA replication-Current Advances, Intech, H. Seligmann ed.
24. Ligasová, A., Koberna K. (2010) Nucleic Acids Res., 38(16):e167
25. Ligasová, A., et al. (2009) J. Struct. Biol., 165(3):204-213
26. Cvačková, Z., et al. (2008) Chromosoma, 117(5):487-497
27. Kalmárová, M., et al. (2007) J. Struct. Biol., 160(1):49-56
28. Cmarko, D., Koberna, K. (2007) Methods Mol. Biol. 369:213-228, J. Kuo ed.
29. Stavreva, D. A., et al. (2006) Mol. Cell Biol., 26:5131-5145
30. Smirnov, E., et al. (2006) Folia Biol., 52:59-70
31. Fidlerová ,H., et al. (2005) J. Cell Biochem., 94:899-916
32. Pliss, A., et al. (2005) J. Cell Biochem., 15:554-565
33. Li, J., et al. (2005) EMBO J., 12:120-127
34. Koberna, K., et al. (2005) J. Cell Biochem., 94(1):126-138
35. Večeřová, J., et al. (2004) Mol. Biol. Cell, 15:4904-4910
36. Raška, I., et al. (2004) Biol. Cell, 96:579-594
37. Malínský, J., et al. (2002) J. Struct. Biol., 140:227-231
38. Koberna, K., et al. (2002) J. Cell Biol., 157:743-748
39. Kopský, V., et al. (2002) Fol. Biol. (Prague), 48:69-72
40. Staněk, D., et al. (2001) Chromosoma, 110(7):460-470
41. Malínský, J., et al. (2001) J. Cell Sci., 114(Pt 4):747-750
42. Koberna, K., et al., (2000) Acta Histochem., 102(1):15-20
43. Melčák, I., et al. (2000) Mol. Biol. Cell., 11(2):497-510
44. Koberna, K., et al. (1999) Chromosoma, 108(5):325-335
45. Koberna, K., et al. (1998) Reprod. Nutr. Dev., 38(1):117-126
46. Schul, W., et al. (1996) EMBO J., 15(11):2883-2892
47. Raška, I., et al. (1995) J. Struct. Biol., 114(1):1-22
48. Raška, I., et al. (1992) Cell Biol. Int. Rep., 16(8):771-789
49. Raška, I., et al. (1989) Exp. Cell Res., 184(1):81-89
50. Raška, I., et al. (1987) Histochem. J., 19(10-11):603

PATENTS                                      

• Czech: 302613; 303230; 303251; 303252; 306187; 307016; 307807; 307304; 307454; 307415; 308519; 308385; 308387, 309218
• European: 3186387, 3395956, 3558526

UTILITY MODELS

• Czech: 28964; 34386; 34442

INDUSTRIAL DESIGN

• Czech: 3668

HONOURS AND AWARDS

2003     Premium of Otto Wichterle for young scientists AS CR