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PhD Club lecture: Orion multiplex immunofluorescence imaging and RareCyte Staining

Dear colleagues,
We would like to invite you to the lecture by Romana Hendrychová called "DNA polymerase α-primase protects BRCA1-deficient cells against ssDNA gaps". The lecture will be on Wednesday June 26, 2024 at 9AM in the IMTM seminary room 1.09.


Precision medicine is critically dependent on better methods of diagnosing and stagingdisease and predicting drug response. Histopathology using hematoxylin and eosin (H&E)-stained tissue remains the primary diagnostic method for cancer. Recently developed highlymultiplexed tissue imaging methods promise to improve research studies and clinical practicewith accurate, spatially resolved single-cell data. One of these is RareCyte's Orion platform, which enables the collection of H&E and highly complex immunofluorescence images fromthe same cells in a whole slide format suitable for diagnostics. This approach enables rapid IFstaining of up to 18 biomarkers in a single step.

The Institute of Clinical and Molecular Pathology, Faculty of Medicine, UPOL, was selectedby RareCyte to loan the platform for 1 year. In cooperation with the staff of this company, ourtask is to make this equipment operational. To date, we have a panel of 8 antibodiesconjugated with immunofluorescent label, comprising: CD3e, CD8a, CD68, CD163, E-cadherin, FAP, FOXP3 and TIGIT. Almost all antibodies work relatively well except for theCD68 marker, for which we are still looking for a way to obtain optimal staining.